|
VU e-Archive >
Undergraduate Honors Research >
Physics and Astronomy Department Honors Program >
High Honors in Physics >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/1803/735
|
| Title: | Reflection and rotation for three dimensional microscopy of live cells |
| Authors: | Wright, Charlie |
| Keywords: | 3d microscopy
reflection
rotation
MPWs |
| Issue Date: | 18-Apr-2008 |
| LC Subjects: | Three-dimensional imaging in biology.
Electron microscopy.
Confocal microscopy.
Cytology -- Technique.
Biological specimens.
Yeast -- Analysis. |
| Abstract: | Confocal scanning laser microscopy and multiphoton microscopy provide 3D data from biological specimens, but with limited z-axis precision. Multiple microscale mirrors can be used to obtain more accurate 3D data on living cells while using classical widefield microscopy. Etched silicon wells coated with aluminum were used to obtain 3D images of budding yeast cells, with information along the z-axis provided by reflections from the angled sides of the well. To supplement measurements obtained with this method, work was also conducted on a system to allow for rotation of a cell attached to a pulled glass tip. Images of a yeast cell obtained from either method can be fit to a simple 3D surface due to the cell’s roughly spheroidal shape, which should provide accurate measurements of the volume of an individual budding yeast cell as it progresses through the cell cycle. |
| URI: | http://hdl.handle.net/1803/735 |
| Appears in Collections: | High Honors in Physics
High Honors in Physics
|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
|
